1uvo
From Proteopedia
STRUCTURE OF THE COMPLEX OF PORCINE PANCREATIC ELASTASE IN COMPLEX WITH CADMIUM REFINED AT 1.85 A RESOLUTION (CRYSTAL A)
Overview
Two series of complete and highly redundant data sets were collected at wavelengths of 1.00 and 2.00 Angstroms on a cadmium derivative of porcine pancreatic elastase (PPE). Radiation damage to the sample was evaluated qualitatively by inspecting consecutive difference electron density maps during the course of the experiment. The nature of the radiation damage was found to be identical at both wavelengths and was localized primarily at the four disulfide bridges of PPE, the cadmium site and the two methionine residues. For a quantitative examination of the radiation damage, the decrease in the peak height of the cadmium ion in various electron density maps was exploited. Again, no significant difference in radiation damage between the two wavelengths was observed. This can be rationalized by considering the wavelength dependencies of the number of diffracted photons versus the number of absorbed photons and the energy deposited in the crystal by the latter.
About this Structure
1UVO is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
On the influence of the incident photon energy on the radiation damage in crystalline biological samples., Weiss MS, Panjikar S, Mueller-Dieckmann C, Tucker PA, J Synchrotron Radiat. 2005 May;12(Pt 3):304-9. Epub 2005 Apr 14. PMID:15840915 Page seeded by OCA on Sat May 3 11:44:51 2008
