2q9t
From Proteopedia
High-resolution structure of the DING protein from Pseudomonas fluorescens
Overview
A recombinant DING protein from Pseudomonas fluorescens has been previously shown to have a phosphate-binding site, and to be mitogenic for human cells. Here we report the three-dimensional structure of the protein, confirming a close similarity to the "Venus flytrap" structure seen in other human and bacterial phosphate-binding proteins. Site-directed mutagenesis confirms the role of a key residue involved in phosphate binding, and that the mitogenic activity is not dependent on this property. Deletion of one of the two hinged domains that constitute the Venus flytrap also eliminates phosphate binding whilst enhancing mitogenic activity.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structure-function relationships in a bacterial DING protein., Ahn S, Moniot S, Elias M, Chabriere E, Kim D, Scott K, FEBS Lett. 2007 Jul 24;581(18):3455-60. Epub 2007 Jun 27. PMID:17612529 Page seeded by OCA on Wed Jun 18 12:03:30 2008
