From Proteopedia

proteopedia linkproteopedia link

Biol 430 Biology Major Partners: Delo Sarr, Mustapha Hussain

spinqualitylabelsall models Glutamine synthase Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Contents 1 Jmol practice 2 Stoichiometry 3 Quarternary Structure Polymerization 4 Active Site 5 Glutamine Synthetase Symmetry

Jmol practice

Backbone with ligand

Labeled Ligands in Chain A

Active Site with Amino Acid Residues

Connection between Chain F residue 63 and Chain G residue 319

Stoichiometry

The stoichiometry of Glutamine Synthetase is as follows: Glutamate + NHз + ATP --> Glutamine + ADP + Pi + H+

In this reaction, Glutamate is used as a major reactant and ATP is used for energy to form Glutamine. It should be noted that along with the enzyme, Me+ which can be either manganese or magnesium is used as a catalyst.

This is however, a 2-step reaction. In the first step of this reaction, an intermediate Υ-glutamyl phosphate is formed. The formation of the intermediate is done by phosphoryl transfer an ATP group Y-carboxylate group of glutamate. In the second step of this reaction, NH4+ attacks the activated intermediate to release the phosphate group yielding Glutamine.

Quarternary Structure Polymerization

(Insert Text Here)

Active Site

(Insert Text Here)

Glutamine Synthetase Symmetry