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- 18:52, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:July22Exp2.png" β (July 12th, 2022, enzymatic assay data for five 200 ππΏ samples of varying 2O14 enzyme concentration plotted as absorbance versus time of reaction. The assay measured the hydrolysis reaction in samples containing uniform PNPA concentrations with varyi)
- 18:49, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:MMdata3.png" β (The plot of approximated velocity versus substrate volume for trial data from the Experiment of December 1st, 2022. The trial used eight 200 ππΏ samples containing 0.000625 mg/mL of 2O14 with PBS buffer, 7.0 pH and 2.0 ππΏ, 6.0 ππΏ, 8.0 π)
- 18:49, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:MMdata2.png" β (The plot of approximated velocity versus substrate volume for trial data from the experiment of November 18th, 2022. The trial used eight 200 ππΏ samples containing 0.000625 mg/mL of 2O14 with PBS buffer, 7.0 pH and 2.0 ππΏ, 6.0 ππΏ, 10.0 π)
- 18:48, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:MMeq.png" β (The Michaelis-Menten Equation.)
- 18:47, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:July22Exp1.png" β (Data for the 200 ππΏ Assay Samples Used for Assay Experiment Conducted on July 12th, 2022.)
- 18:45, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:MMdata1.png" β (The plot of approximated velocity versus substrate volume for trial data from the experiment of November 18th, 2022. The trial used eight 200 ππΏ samples containing 0.000625 mg/mL of 2O14 with PBS buffer, 7.0 pH and 2.0 ππΏ, 6.0 ππΏ, 10.0)
- 18:01, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:Bradforddialyzedmeasurements.png" β (Data of the Absorbance Measurements for Dilutions of the Purified, Dialyzed 2O14 Sample.)
- 17:59, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:PNPAreaction.png" β (Hydrolysis reaction of p-nitrophenyl acetate (PNPA).)
- 17:46, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:Bradfordplot.png" β (Microsoft Excel plot of absorbance versus concentration of six BSA standards from Bradford assay. The linear regression was adjusted from a larger data set to provide an appropriate slope intercept equation. The slope intercept equation was calculated b)
- 17:00, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:SDSPAGE.png" β (SDS-PAGE analysis of fractions eluted from Ni-NTA agarose column for 2O14 purification. The gel was stained with Coomasie blue. Elution 1, 2, 3, 4, and 5 are the fractions that were expected to contain the 2O14 protein. According to the standard, these )
- 16:51, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:Growthplate.png" β (2O14 transformation plate growths. The transformation plate was made with LB medium, agar, and contained with 100 ππ/ππΏ ampicillin. )
- 16:31, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:Insilicosum2.png" β (Image created in PyMOL molecular viewing software showing the structure of 2o14 with its five residues SER 171, ASP 339, HIS 342, GLY 209, and ASN 241 that belong to the proposed active site colored in yellow. )
- 16:27, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:Insilicosum1.png" β (Summary of most significant computational results for query 2O14 used to form hypothesis about 2O14's function.)
- 16:11, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:MCSA7.png" β (Hydrolysis of the 2-acetyl-1-alkyl-sn-glycero-3-phosphocoholine substrate from the reaction catalyzed by 1bwp. The bond circled in green is the ester bond that is cleaved. Image was created using Microsoft Paint and ChemDraw.)
- 16:06, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:MCSA6.png" β (Enzyme reaction catalyzed by 1j00, EC 3.1.1.5, lysophospholipase. The scheme was adapted from M-CSA and created in ChemDraw)
- 16:06, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:MCSA5.png" β (Enzyme reaction catalyzed by 1pp4, EC 3.1.1.86, rhamnogalacturonan acetylesterase. The scheme was adapted from M-CSA and created in ChemDraw.)
- 15:59, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:MCSA4.png" β (Enzyme reaction catalyzed by 1bwp, EC 3.1.1.47, 1-alkyl-2-acetylglycerophosphocholine esterase. The scheme was adapted from M-CSA and created in ChemDraw <ref name="Chemdraw"> Revvity Signals Software. ChemDraw | Revvity Signals Software. https://revvity)
- 13:15, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:MCSA3.png" β (M-CSA mechanism description information for 1avf and 1bbs, EC 3.4.23, aspartic peptidase)
- 13:14, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:MCSA2.png" β ( M-CSA mechanism description information for 1bwp, EC 3.1.1.47, 1-alkyl-2-acetylglycerophosphocholine esterase <ref name="MCSA"> . Ribeiro, A. J. M.; Holliday, G. L.; Furnham, N.; Tyzack, J. D.; Ferris, K.; Thornton, J. M. Mechanism and Catalytic Site Atl)
- 13:13, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:MCSA1.png" β (Table 3. EC numbers of the six most significant SPRITE result enzymes with definitions from M-CSA)
- 12:50, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:SPRITE4.png" β (SPRITE catalytic motif residue alignment image for a 1pp4 entry β1pp4_c00β and 2O14. 2O14βs residues are colored yellow. 1pp4βs residues are colored green. In the matches column 1pp4βs residues are listed first and 2O14βs residues are list)
- 12:49, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:SPRITE3.png" β (SPRITE catalytic motif residue alignment image for a 1bwp entry β1bwp_c00β and 2O14. 2O14βs residues are colored yellow. 1bwpβs residues are colored green. In the matches column 1bwpβs residues are listed first and 2O14βs residues are list)
- 12:27, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:SPRITE2.png" β ( 2O14 residues that align with the catalytic site residues of the six most significant enzyme results provided by SPRITE.)
- 12:24, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:SPRITE1.png" β (SPRITE results having scores indicating the most significant catalytic motif alignment with query 2O14.)
- 11:42, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:DALI2.png" β ( DALI results #20-50 for query 2O14 viewed during May 2023. The information of each result entry is listed from left to right as follows: βNo:β, βChainβ, βZβ, βrmsdβ, βlaliβ, βnresβ, β%idβ, βPDBβ, and βDescriptionβ )
- 11:39, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:DALI1.png" β (DALI results #1-19 for query 2O14 viewed during May 2023. The information of each result entry is listed from left to right as follows: βNo:β, βChainβ, βZβ, βrmsdβ, βlaliβ, βnresβ, β%idβ, βPDBβ, and βDescriptionβ <ref )
- 11:23, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:Interproresults.png" β (InterPro Results for Query Using 2O14βs FASTA Sequence as Seen in May 2023.)
- 11:17, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:Interproresults.PNG" β (InterPro Results for Query Using 2O14βs FASTA Sequence Shown in May 2023. )
- 11:05, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:BLASTpdomainentry.png" β (BLAST graphic showing conserved domain and superfamily entries for query 2O14 that was viewed during May 2023.)
- 10:59, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:BLASTpdomainentry.PNG" β (BLAST graphic showing conserved domain and superfamily entries for query 2O14 that was viewed during May 2023.)
- 10:39, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:Blastpparameters.png" β ( Figure 2. BLAST Parameters of the Query 2O14 Conducted on May 28th, 2023 <ref> Sayers, E. W.; Bolton, E. E.; Brister, J. R.; Canese, K.; Chan, J.; Comeau, D. D.; Farrell, C. M.; Feldgarden, M.; Fine, A. M.; Funk, K.; et al. Database resources of the Nat)
- 10:35, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:Blastp parameters.PNG" β (Figure 2. BLAST Parameters of the Query 2O14 Conducted on May 28th, 2023 <ref> Sayers, E. W.; Bolton, E. E.; Brister, J. R.; Canese, K.; Chan, J.; Comeau, D. D.; Farrell, C. M.; Feldgarden, M.; Fine, A. M.; Funk, K.; et al. Database resources of the Nati)
- 10:19, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded a new version of "Image:Workflow1.PNG" β (Flowchart showing the methodological approach used for functional characterization of 2O14. Order of methods is read from top to bottom and left to right.)
- 10:16, 10 March 2024 Gregg T. Holliday (Talk | contribs) uploaded "Image:Workflow1.PNG" β (Flowchart showing the methodological approach used for functional characterization of 2O14. Order of methods is read from top to bottom and left to right.)
