User:Laura Fountain/Chloride Ion Channel
From Proteopedia
CLIC1: A Chloride Ion Channel
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CLIC1 (NCC27) is a member of the highly conserved class of chloride ion channels that exist in both soluble and integral membrane forms. Purified CLIC1 can integrate into synthetic lipid bilayers forming a chloride channel with similar properties to those observed in vivo. The structure of the soluble form of CLIC1 has been determined at 1.4-A resolution, and is shown to the right. Integration of CLIC1 into the membrane is likely to require a major structural rearrangement, probably of the N-domain (), with the putative transmembrane helix arising from residues in the vicinity of the redox-active site.[1]
About this Structure
1K0O is a 2 chains structure of sequences from Homo sapiens. The protein is monomeric and structurally homologous to the glutathione S-transferase superfamily, and it has a redox-active site resembling glutaredoxin. The structure of the complex of CLIC1 with glutathione shows that glutathione occupies the redox-active site, which is adjacent to an open, elongated slot lined by basic residues. This structure indicates that CLIC1 is likely to be controlled by redox-dependent processes.[2]
References
[1]Harrop SJ, DeMaere MZ, Fairlie WD, Reztsova T, Valenzuela SM, Mazzanti M, Tonini R, Qiu MR, Jankova L, Warton K, Bauskin AR, Wu WM, Pankhurst S, Campbell TJ, Breit SN, Curmi PM. Crystal structure of a soluble form of the intracellular chloride ion channel CLIC1 (NCC27) at 1.4-A resolution. J Biol Chem. 2001 Nov 30;276(48):44993-5000. Epub 2001 Sep 10. PMID:11551966
