1kxk
From Proteopedia
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Crystal Structure of a RNA Molecule Containing Domain 5 and 6 of the Yeast ai5g Group II Self-splicing Intron
Overview
Group II self-splicing introns catalyze autoexcision from precursor RNA, transcripts by a mechanism strikingly similar to that of the spliceosome, an RNA-protein assembly responsible for splicing together the, protein-coding parts of most eukaryotic pre-mRNAs. Splicing in both cases, initiates via nucleophilic attack at the 5' splice site by the 2' OH of a, conserved intron adenosine residue, creating a branched (lariat), intermediate. Here, we describe the crystal structure at 3.0 A resolution, of a 70-nucleotide RNA containing the catalytically essential domains 5, and 6 of the yeast ai5gamma group II self-splicing intron, revealing an, unexpected two-nucleotide bulged structure around the branch-point, adenosine in domain 6.
About this Structure
1KXK is a Protein complex structure of sequences from [1] with MG as ligand. Full crystallographic information is available from OCA.
Reference
Structural insights into group II intron catalysis and branch-site selection., Zhang L, Doudna JA, Science. 2002 Mar 15;295(5562):2084-8. Epub 2002 Feb 21. PMID:11859154
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