1d14
From Proteopedia
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STRUCTURE OF 11-DEOXYDAUNOMYCIN BOUND TO DNA CONTAINING A PHOSPHOROTHIOATE
Overview
The anthracyclines form an important family of cancer chemotherapeutic, agents with a strong dependence of clinical properties on minor, differences in chemical structure. We describe the X-ray crystallographic, solution of the three-dimensional structure of the anthracycline, 11-deoxydaunomycin plus d(CGTsACG). In this complex, two drug molecules, bind to each hexamer duplex. Both the drug and the DNA are covalently, modified in this complex in contrast with the three previously reported, DNA-anthracycline complexes. In the 11-deoxydaunomycin complex the 11, hydroxyl group is absent and a phosphate oxygen at the TpA step has been, replaced by a sulfur atom leading to a phosphorothioate with absolute, stereochemistry R. Surprisingly, removal of a hydroxyl group from the 11, position does not alter the relative orientation of the intercalated, chromophore. However, it appears that the phosphorothioate modification, influenced the crystallization and caused the, 11-deoxydaunomycin-d(CGTsACG) complex to crystallize into a different, lattice (space group P2) with different lattice contacts and packing, forces than the non-phosphorothioated DNA-anthracycline complexes (space, group P4(1)2(1)2). In the minor groove of the DNA, the unexpected position, of the amino-sugar of 11-deoxydaunomycin supports the hypothesis that in, solution the position of the amino sugar is dynamic.
About this Structure
1D14 is a Protein complex structure of sequences from [1] with DM3 as ligand. Full crystallographic information is available from OCA.
Reference
Structure of 11-deoxydaunomycin bound to DNA containing a phosphorothioate., Williams LD, Egli M, Ughetto G, van der Marel GA, van Boom JH, Quigley GJ, Wang AH, Rich A, Frederick CA, J Mol Biol. 1990 Sep 20;215(2):313-20. PMID:2152325
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