1d90
From Proteopedia
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REFINED CRYSTAL STRUCTURE OF AN OCTANUCLEOTIDE DUPLEX WITH I.T MISMATCHED BASE PAIRS
Overview
The structure of the synthetic deoxyoctamer d(GGIGCTCC) has been, determined by single crystal X-ray diffraction techniques to a resolution, of 1.7A. The sequence crystallises in space group P6(1), with unit cell, dimensions a = b = 45.07, c = 45.49A. The refinement converged with a, crystallographic residual R = 0.14 and the location of 81 solvent, molecules. The octamer forms an A-DNA duplex with 6 Watson-Crick (G.C), base pairs and 2 inosine-thymine (I.T) pairs. Refinement of the structure, shows it to be essentially isomorphous with that reported for d(GGGGCTCC), with the mispairs adopting a "wobble" conformation. Conformational, parameters and base stacking interactions are compared to those for the, native duplex d(GGGGCCCC) and other similar sequences. A rationale for the, apparent increased crystal packing efficiency and lattice stability of the, I.T octamer is given.
About this Structure
1D90 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Refined crystal structure of an octanucleotide duplex with I.T. mismatched base pairs., Cruse WB, Aymani J, Kennard O, Brown T, Jack AG, Leonard GA, Nucleic Acids Res. 1989 Jan 11;17(1):55-72. PMID:2911488
Page seeded by OCA on Sun Nov 25 02:46:40 2007
Categories: Protein complex | Aymani, J. | Brown, T. | Cruse, W.B.T. | Jack, A.G.C. | Kennard, O. | Leonard, G.A. | A-dna | Double helix | Mismatched | Modified
