1pqt
From Proteopedia
|
REFINEMENT OF d(GCGAAGC) HAIRPIN STRUCTURE USING ONE- AND TWO-BOND RESIDUAL DIPOLAR COUPLINGS
Overview
The structure of the 13C,15N-labeled d(GCGAAGC) hairpin, as determined by, NMR spectroscopy and refined using molecular dynamics with NOE-derived, distances, torsion angles, and residual dipolar couplings (RDCs), is, presented. Although the studied molecule is of small size, it is, demonstrated that the incorporation of diminutive RDCs can significantly, improve local structure determination of regions undefined by the, conventional restraints. Very good correlation between the experimental, and back-calculated small one- and two-bond 1H-13C, 1H-15N, 13C-13C and, 13C-15N coupling constants has been attained. The final structures clearly, show typical features of the miniloop architecture. The structure is, discussed in context of the extraordinary stability of the d(GCGAAGC), hairpin, which originates from a complex interplay between the aromatic, base stacking and hydrogen bonding interactions.
About this Structure
1PQT is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Refinement of d(GCGAAGC) hairpin structure using one- and two-bond residual dipolar couplings., Padrta P, Stefl R, Kralik L, Zidek L, Sklenar V, J Biomol NMR. 2002 Sep;24(1):1-14. PMID:12449414
Page seeded by OCA on Sun Nov 25 02:53:22 2007
Categories: Protein complex | Padrta, P. | Sklenar, V. | Stefl, R. | Zidek, L. | Dna | Ga mismatch | Hairpin
