1ddp

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models 1ddp Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK

Overview

The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin, or cis-DDP) reacts with DNA, cross-linking two purine residues through the, N7 atoms, which reside in the major groove in B-form DNA. The solution, structure of the short duplex [d(CAT-AGCTATG)]2 cross-linked at the GC:GC, site was determined by nuclear magnetic resonance (NMR). The, deoxyguanosine-bridging cis-diammineplatinum(II) lies in the minor groove, and the complementary deoxycytidines are extrahelical. The double helix is, locally reversed to a left-handed form, and the helix is unwound and bent, toward the minor groove. These findings were independently confirmed by, results from a phase-sensitive gel electrophoresis bending assay. The NMR, structure differs markedly from previously proposed models but accounts, for the chemical reactivity, the unwinding, and the bending of cis-DDP, interstrand cross-linked DNA and may be important in the formation and, repair of these cross-links in chromatin.

About this Structure

1DDP is a Protein complex structure of sequences from [1] with CPT as ligand. Full crystallographic information is available from OCA.

Reference

Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:8525382

Page seeded by OCA on Sun Nov 25 03:01:16 2007