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1hv6
From Proteopedia
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CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.
Overview
The structure of A1-III from a Sphingomonas species A1 complexed with a, trisaccharide product, (4-deoxy-l-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid), was determined by X-ray crystallography at 2.0 A with an R-factor of 0.16., The final model of the complex form comprising 351 amino acid residues, 245 water molecules, one sulfate ion and one trisaccharide product, exhibited a C(alpha) r.m.s.d. value of 0.154 A with the reported apo form, of the enzyme. The trisaccharide was bound in the active cleft at subsites, -3 approximately -1 from the non-reducing end by forming several hydrogen, bonds and van der Waals interactions with protein atoms. The catalytic, residue was estimated to be Tyr246, which existed between subsites -1 and, +1 based on a mannuronic acid model oriented at subsite +1.
About this Structure
1HV6 is a Single protein structure of sequence from Sphingomonas species with SO4 as ligand. Active as Poly(beta-D-mannuronate) lyase, with EC number 4.2.2.3 Full crystallographic information is available from OCA.
Reference
Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution., Yoon HJ, Hashimoto W, Miyake O, Murata K, Mikami B, J Mol Biol. 2001 Mar 16;307(1):9-16. PMID:11243798
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