1dx8

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RUBREDOXIN FROM GUILLARDIA THETA

Overview

The rubredoxin from the cryptomonad Guillardia theta is one of the first, examples of a rubredoxin encoded in a eukaryotic organism. The structure, of a soluble zinc-substituted 70-residue G. theta rubredoxin lacking the, membrane anchor and the thylakoid targeting sequence was determined by, multidimensional heteronuclear NMR, representing the first, three-dimensional (3D) structure of a eukaryotic rubredoxin. For the, structure calculation a strategy was applied in which information about, hydrogen bonds was directly inferred from a long-range HNCO experiment, and the dynamics of the protein was deduced from heteronuclear nuclear, Overhauser effect data and exchange rates of the amide protons. The, structure is well defined, exhibiting average root-mean-square deviations, of 0.21 A for the backbone heavy atoms and 0.67 A for all heavy atoms of, residues 7-56, and an increased flexibility toward the termini. The, structure of this core fold is almost identical to that of prokaryotic, rubredoxins. There are, however, significant differences with respect to, the charge distribution at the protein surface, suggesting that G. theta, rubredoxin exerts a different physiological function compared to the, structurally characterized prokaryotic rubredoxins. The amino-terminal, residues containing the putative signal peptidase recognition/cleavage, site show an increased flexibility compared to the core fold, but still, adopt a defined 3D orientation, which is mainly stabilized by nonlocal, interactions to residues of the carboxy-terminal region. This orientation, might reflect the structural elements and charge pattern necessary for, correct signal peptidase recognition of the G. theta rubredoxin precursor.

About this Structure

1DX8 is a Single protein structure of sequence from Guillardia theta with ZN as ligand. Full crystallographic information is available from OCA.

Reference

Solution structure of a zinc substituted eukaryotic rubredoxin from the cryptomonad alga Guillardia theta., Schweimer K, Hoffmann S, Wastl J, Maier UG, Rosch P, Sticht H, Protein Sci. 2000 Aug;9(8):1474-86. PMID:10975569

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