1ybk

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Image:1ybk.gif

1ybk, resolution 1.45Å

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RHCC cocrystallized with CAPB

Overview

Crystals of excellent quality are a prerequisite for high-resolution X-ray, data. However, in refinement protocols of crystallization conditions it is, often difficult to obtain the right combination of, for example, protein, concentration, drop size, temperature and additives. A novel approach for, optimizing crystal contacts in a most favourable fashion by performing, crystallization setups with the zwitterionic surfactant, cocoamidoproylbetaine (CAPB) is introduced. In the presence of this, surfactant, highly diffracting crystals were obtained. Here, data from a, right-handed coiled coil (RHCC) in complex with CAPB at 1.4 A resolution, are presented. The addition of CAPB using otherwise identical, crystallization conditions and the same X-ray source caused an improvement, in resolution from 2.9 to 1.4 A.

About this Structure

1YBK is a Single protein structure of sequence from Staphylothermus marinus with SO4 and BEQ as ligands. Full crystallographic information is available from OCA.

Reference

Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB)., Ozbek S, Muller JF, Figgemeier E, Stetefeld J, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):477-80. Epub 2005, Mar 24. PMID:15805603

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