1ygm
From Proteopedia
|
NMR structure of Mistic
Overview
Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental, bottlenecks in obtaining both sufficient yields of protein and ordered, crystals. Mistic is an unusual Bacillus subtilis integral membrane protein, that folds autonomously into the membrane, bypassing the cellular, translocon machinery. Using paramagnetic probes, we determined by nuclear, magnetic resonance (NMR) spectroscopy that the protein forms a helical, bundle with a surprisingly polar lipid-facing surface. Additional, experiments suggest that Mistic can be used for high-level production of, other membrane proteins in their native conformations, including many, eukaryotic proteins that have previously been intractable to bacterial, expression.
About this Structure
1YGM is a Single protein structure of sequence from Bacillus subtilis subsp. subtilis str. 168. Full crystallographic information is available from OCA.
Reference
NMR structure of Mistic, a membrane-integrating protein for membrane protein expression., Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S, Science. 2005 Feb 25;307(5713):1317-21. PMID:15731457
Page seeded by OCA on Sun Nov 25 04:06:33 2007
