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1uk8
From Proteopedia
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Crystal structure of a meta-cleavage product hydrolase (CumD) complexed with n-valerate
Overview
Meta-cleavage product hydrolase (MCP-hydrolase) is one of the key enzymes, in the microbial degradation of aromatic compounds. MCP-hydrolase produces, 2-hydroxypenta-2,4-dienoate and various organic acids, according to the C6, substituent of the substrate. Comprehensive analysis of the substrate, specificity of the MCP-hydrolase from Pseudomonas fluorescens IP01 (CumD), was carried out by determining the kinetic parameters for nine substrates, and crystal structures complexed with eight cleavage products. CumD, preferred substrates with long non-branched C6 substituents, but did not, effectively hydrolyze a substrate with a phenyl group. Superimposition of, the complex structures indicated that benzoate was bound in a, significantly different direction than other aliphatic cleavage products., The directions of the bound organic acids appeared to be related with the, k(cat) values of the corresponding substrates. The Ile139 and Trp143, residues on helix alpha4 appeared to cause steric hindrance with the, aromatic ring of the substrate, which hampers base-catalyzed attack by, water.
About this Structure
1UK8 is a Single protein structure of sequence from Pseudomonas fluorescens with LEA as ligand. Active as 2-hydroxymuconate-semialdehyde hydrolase, with EC number 3.7.1.9 Full crystallographic information is available from OCA.
Reference
A series of crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with various cleavage products., Fushinobu S, Jun SY, Hidaka M, Nojiri H, Yamane H, Shoun H, Omori T, Wakagi T, Biosci Biotechnol Biochem. 2005 Mar;69(3):491-8. PMID:15784976
Page seeded by OCA on Sun Nov 25 04:06:49 2007
Categories: 2-hydroxymuconate-semialdehyde hydrolase | Pseudomonas fluorescens | Single protein | Fushinobu, S. | Hidaka, M. | Jun, S.Y. | Nojiri, H. | Omori, T. | Shoun, H. | Wakagi, T. | Yamane, H. | LEA | Alpha/beta-hydrolase | Aromatic compounds | Beta-ketolase | Cumene | Cumene degradation | Isopropylbenzene | Meta-cleavage compound hydrolase | Pcb | Polychlorinated biphenyl degradation | Pseudomonas fluorescens ip01 | Substrate specificity
