1ukm

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models 1ukm, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of EMS16, an Antagonist of collagen receptor integrin alpha2beta1 (GPIa/IIa)

Overview

Snake venoms contain a number of hemostatically active C-type lectin-like, proteins (CLPs), which affect the blood coagulation system, endothelial, cells, and platelets. CLPs have broad similarities in structure and, possess distinct biological functions. EMS16, a CLP from Echis, multisquamatus venom, which is a potent and selective inhibitor of the, collagen receptor, glycoprotein Ia/IIa (integrin alpha2beta1), has been, used in the present study to examine structure-function relationships in, venom CLPs by X-ray crystallography. The structure of EMS16, determined at, a resolution of 1.9 A, revealed a heterodimer involved with domain, swapping of the central loop as observed in the structures of other CLPs., A part of the glycan was observed and identified as N-acetyl-D-glucosamine, (GlcNAc) in the electron density map at Asn21 of subunit B, an expected, glycosylation site. EMS16 had a unique, positively charged electrostatic, potential patch on the concave surface that may qualify as a site for, interaction with the I-domain of the glycoprotein Ia/IIa.

About this Structure

1UKM is a Protein complex structure of sequences from Echis multisquamatus with NAG, CL and GOL as ligands. Full crystallographic information is available from OCA.

Reference

Structural characterization of EMS16, an antagonist of collagen receptor (GPIa/IIa) from the venom of Echis multisquamatus., Horii K, Okuda D, Morita T, Mizuno H, Biochemistry. 2003 Nov 4;42(43):12497-502. PMID:14580195

Page seeded by OCA on Sun Nov 25 04:08:38 2007