1mfy

From Proteopedia

SOLUTION STRUCTURE OF INFLUENZA A VIRUS C4 PROMOTER

Overview

The influenza A virus promoter is recognized by the influenza A virus, RNA-dependent RNA polymerase, and directs both transcription and, replication of the viral RNA genome. Within the sequence of this promoter, flu strains exhibit a natural, unique variation, either a U or a C, at the, fourth position from the 3' end. Promoters that contain a C residue (C4, promoter), which are invariably found in genome segments that encode the, three RNA polymerase subunits (PB1, PB2 and PA), down-regulate, transcription but activate genome replication. Here, we have determined, the structure of the C4 promoter by NMR spectroscopy and compared it with, the structure of the U4 promoter, which was determined previously. The, structure of the internal loop in the C4 promoter is similar to that of, the U4 promoter. However, the terminal stem of the C4 promoter is, strikingly different from that of the U4 promoter. These structural data, suggest that the internal loop is important for polymerase binding to the, promoter, and the terminal stem is crucial for differential regulation of, transcription and replication.

About this Structure

1MFY is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

A single-nucleotide natural variation (U4 to C4) in an influenza A virus promoter exhibits a large structural change: implications for differential viral RNA synthesis by RNA-dependent RNA polymerase., Lee MK, Bae SH, Park CJ, Cheong HK, Cheong C, Choi BS, Nucleic Acids Res. 2003 Feb 15;31(4):1216-23. PMID:12582241

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