1gk4
From Proteopedia
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HUMAN VIMENTIN COIL 2B FRAGMENT (CYS2)
Contents |
Overview
Intermediate filaments (IFs) are key components of the cytoskeleton in, higher eukaryotic cells. The elementary IF 'building block' is an, elongated coiled-coil dimer consisting of four consecutive alpha-helical, segments. The segments 1A and 2B include highly conserved sequences and, are critically involved in IF assembly. Based on the crystal structures of, three human vimentin fragments at 1.4-2.3 A resolution (PDB entries 1gk4, 1gk6 and 1gk7), we have established the molecular organization of these, two segments. The fragment corresponding to segment 1A forms a single, amphipatic alpha-helix, which is compatible with a coiled-coil geometry., While this segment might yield a coiled coil within an isolated dimer, monomeric 1A helices are likely to play a role in specific dimer-dimer, interactions during IF assembly. The 2B segment reveals a double-stranded, coiled coil, which unwinds near residue Phe351 to accommodate a 'stutter'., A fragment containing the last seven heptads of 2B interferes heavily with, IF assembly and also transforms mature vimentin filaments into a new kind, of structure. These results provide the first insight into the, architecture and functioning of IFs at the atomic level.
Disease
Known disease associated with this structure: Inflammatory response, modulation of OMIM:[607918]
About this Structure
1GK4 is a Single protein structure of sequence from Homo sapiens with ACT as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Conserved segments 1A and 2B of the intermediate filament dimer: their atomic structures and role in filament assembly., Strelkov SV, Herrmann H, Geisler N, Wedig T, Zimbelmann R, Aebi U, Burkhard P, EMBO J. 2002 Mar 15;21(6):1255-66. PMID:11889032
Page seeded by OCA on Tue Dec 18 15:22:11 2007
