2bgg
From Proteopedia
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THE STRUCTURE OF A PIWI PROTEIN FROM ARCHAEOGLOBUS FULGIDUS COMPLEXED WITH A 16NT SIRNA DUPLEX.
Overview
RNA interference and related RNA silencing phenomena use short antisense, guide RNA molecules to repress the expression of target genes. Argonaute, proteins, containing amino-terminal PAZ (for PIWI/Argonaute/Zwille), domains and carboxy-terminal PIWI domains, are core components of these, mechanisms. Here we show the crystal structure of a Piwi protein from, Archaeoglobus fulgidus (AfPiwi) in complex with a small interfering RNA, (siRNA)-like duplex, which mimics the 5' end of a guide RNA strand bound, to an overhanging target messenger RNA. The structure contains a highly, conserved metal-binding site that anchors the 5' nucleotide of the guide, RNA. The first base pair of the duplex is unwound, separating the 5', nucleotide of the guide from the complementary nucleotide on the target, strand, which exits with the 3' overhang through a short channel. The, remaining base-paired nucleotides assume an A-form helix, accommodated, within a channel in the PIWI domain, which can be extended to place the, scissile phosphate of the target strand adjacent to the putative slicer, catalytic site. This study provides insights into mechanisms of target, mRNA recognition and cleavage by an Argonaute-siRNA guide complex.
About this Structure
2BGG is a Single protein structure of sequence from Archaeoglobus fulgidus with MN as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural insights into mRNA recognition from a PIWI domain-siRNA guide complex., Parker JS, Roe SM, Barford D, Nature. 2005 Mar 31;434(7033):663-6. PMID:15800628
Page seeded by OCA on Tue Dec 18 18:45:58 2007
Categories: Archaeoglobus fulgidus | Single protein | Barford, D. | Parker, J.S. | Roe, S.M. | MN | Argonaute | Piwi domain | Protein/rna complex | Risc | Rnai
