2br6

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spinqualitylabelsall models 2br6, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF QUORUM-QUENCHING N-ACYL HOMOSERINE LACTONE LACTONASE

Overview

In many Gram-negative bacteria, including a number of pathogens such as, Pseudomonas aeruginosa and Erwinia carotovora, virulence factor production, and biofilm formation are linked to the quorum-sensing systems that use, diffusible N-acyl-L-homoserine lactones (AHLs) as intercellular messenger, molecules. A number of organisms also contain genes coding for lactonases, that hydrolyze AHLs into inactive products, thereby blocking the, quorum-sensing systems. Consequently, these enzymes attract intense, interest for the development of antiinfection therapies. However, the, catalytic mechanism of AHL-lactonase is poorly understood and subject to, controversy. We here report a 2.0-angstroms resolution structure of the, AHL-lactonase from Bacillus thuringiensis and a 1.7-angstroms crystal, structure of its complex with L-homoserine lactone. Despite limited, sequence similarity, the enzyme shows remarkable structural similarities, to glyoxalase II and RNase Z proteins, members of the, metallo-beta-lactamase superfamily. We present experimental evidence that, AHL-lactonase is a metalloenzyme containing two zinc ions involved in, catalysis, and we propose a catalytic mechanism for bacterial, metallo-AHL-lactonases.

About this Structure

2BR6 is a Single protein structure of sequence from Bacillus thuringiensis with ZN, HSL and GOL as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase., Kim MH, Choi WC, Kang HO, Lee JS, Kang BS, Kim KJ, Derewenda ZS, Oh TK, Lee CH, Lee JK, Proc Natl Acad Sci U S A. 2005 Dec 6;102(49):17606-11. Epub 2005 Nov 28. PMID:16314577

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