108d
From Proteopedia
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THE SOLUTION STRUCTURE OF A DNA COMPLEX WITH THE FLUORESCENT BIS INTERCALATOR TOTO DETERMINED BY NMR SPECTROSCOPY
Overview
We have used two-dimensional 1H NMR spectroscopy to determine the solution, structure of the DNA oligonucleotide d(5'-CGCTAGCG-3')2 complexed with the, bis-intercalating dye, 1,1'-(4,4,8,8-tetramethyl-4,8-diazaundecamethylene)bis[4-(3-methyl -2,3-, dihydrobenzo-1,3-thiazolyl-2-methylidene)qui nolinium] tetraiodide (TOTO)., The determination of the structure was based on total relaxation matrix, analysis of the NOESY cross-peak intensities using the program MARDIGRAS., Improved procedures to consider the experimental "noise" in NOESY spectra, during these calculations have been employed. The NOE-derived distance, restraints were applied in restrained molecular dynamics calculations., Twenty final structures each were generated for the TOTO complex from both, A-form and B-form dsDNA ... [(full description)]
About this Structure
108D is a [Protein complex] structure of sequences from [[1]] with TOT as [ligand]. Full crystallographic information is available from [OCA].
Reference
Solution structure of a DNA complex with the fluorescent bis-intercalator TOTO determined by NMR spectroscopy., Spielmann HP, Wemmer DE, Jacobsen JP, Biochemistry. 1995 Jul 11;34(27):8542-53. PMID:7612596
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