2ixs
From Proteopedia
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STRUCTURE OF SDAI RESTRICTION ENDONUCLEASE
Overview
Rare-cutting restriction enzymes are important tools in genome analysis., We report here the crystal structure of SdaI restriction endonuclease, which is specific for the 8 bp sequence CCTGCA/GG ("/" designates the, cleavage site). Unlike orthodox Type IIP enzymes, which are single domain, proteins, the SdaI monomer is composed of two structural domains. The N, domain contains a classical winged helix-turn-helix (wHTH) DNA binding, motif, while the C domain shows a typical restriction endonuclease fold., The active site of SdaI is located within the C domain and represents a, variant of the canonical PD-(D/E)XK motif. SdaI determinants of sequence, specificity are clustered on the recognition helix of the wHTH motif at, the N domain. The modular architecture of SdaI, wherein one domain, mediates DNA binding while the other domain is predicted to catalyze, hydrolysis, distinguishes SdaI from previously characterized restriction, enzymes interacting with symmetric recognition sequences.
About this Structure
2IXS is a Single protein structure of sequence from Streptomyces diastaticus with SO4, EPE and TRS as ligands. Active as Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The crystal structure of the rare-cutting restriction enzyme SdaI reveals unexpected domain architecture., Tamulaitiene G, Jakubauskas A, Urbanke C, Huber R, Grazulis S, Siksnys V, Structure. 2006 Sep;14(9):1389-400. PMID:16962970
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