2jmh
From Proteopedia
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NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis
Overview
Blo t 5 is the major allergen from Blomia tropicalis mites and shows, strong IgE reactivity with up to 90% of asthmatic and rhinitis patients', sera. The NMR solution structure of Blo t 5 comprises three long alpha, helices, forming a coiled-coil, triple-helical bundle with a left-handed, twist. TROSY-NMR experiments were used to study Blo t 5 interaction with, the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope, comprises two closely spaced surfaces, I and II, connected by a sharp turn, and bearing critical residues Asn46 and Lys47 on one surface, and Lys54, and Arg57 on the other. This discontinuous epitope overlaps with the human, IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to, undergo conformational exchange in the millisecond to microsecond range., The results presented are critical for the design of a hypoallergenic Blo, t 5 for efficacious immunotherapy of allergic diseases.
About this Structure
2JMH is a Single protein structure of sequence from Blomia tropicalis. Full crystallographic information is available from OCA.
Reference
Roles of Structure and Structural Dynamics in the Antibody Recognition of the Allergen Proteins: An NMR Study on Blomia tropicalis Major Allergen., Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH, Structure. 2008 Jan;16(1):125-36. PMID:18184590
Page seeded by OCA on Wed Jan 23 10:39:13 2008
Categories: Blomia tropicalis | Single protein | Chang, C. | Chua, K. | Huang, T. | Kuo, I. | Naik, M.T. | Allergen | Blo t 5 | Dust mites | Group 5
