2qeo
From Proteopedia
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Crystal Structure of Anopheles gambiae D7R4-norepinephrine complex
Overview
The D7-related (D7r) proteins of the malaria vector Anopheles gambiae have, been shown to bind the biogenic amines serotonin, norepinephrine, and, histamine with high affinity. One member of the group (D7r1 or hamadarin), has also been shown to have an anticoagulant/antikinin activity. To, understand the mechanistic details of its antihemostatic/anti-inflammatory, effects, we have determined the crystal structure of one member of this, group, D7r4, along with the structures of ligand complexes with serotonin, tryptamine, histamine, and norepinephrine. The D7 fold consists of an, arrangement of eight alpha-helices stabilized by three disulfide bonds., The structure is similar to those of the arthropod odorant-binding, proteins, a relationship that had been predicted based on sequence, comparisons. Although odorant-binding proteins commonly have six, alpha-helices, D7r4 has eight, resulting in significantly different, positioning and structure of the ligand binding pocket. The pocket itself, is lined by hydrophobic side chains along with polar and charged groups, oriented to form hydrogen bonds with the aliphatic amino group and with, groups on the aromatic portions of the ligands. These structures, along, with accompanying mutagenesis studies, have allowed us to identify, critical residues for biogenic amine binding and to predict which members, of the large D7 protein family found in blood-feeding nematocerous Diptera, will function as biogenic amine-binding proteins.
About this Structure
2QEO is a Single protein structure of sequence from Anopheles gambiae with as ligand. Full crystallographic information is available from OCA.
Reference
The Crystal Structure of D7r4, a Salivary Biogenic Amine-binding Protein from the Malaria Mosquito Anopheles gambiae., Mans BJ, Calvo E, Ribeiro JM, Andersen JF, J Biol Chem. 2007 Dec 14;282(50):36626-33. Epub 2007 Oct 9. PMID:17928288
Page seeded by OCA on Wed Jan 23 11:02:39 2008
