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2rji
From Proteopedia
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Malarial EBA-175 region VI crystallographic structure reveals a KIX-like binding interface
Overview
The malaria parasite proliferates in the bloodstream of its vertebrate, host by invading and replicating within erythrocytes. To achieve, successful invasion, a number of discrete and essential events need to, take place at the parasite-host cell interface. Erythrocyte-binding, antigen 175 (EBA-175) is a member of a family of Plasmodium falciparum, erythrocyte-binding proteins involved in the formation of a tight, junction, a necessary step in invasion. Here we present the crystal, structure of EBA-175 region VI (rVI), a cysteine-rich domain that is, highly conserved within the protein family and is essential for EBA-175, trafficking. The structure was solved by selenomethionine, single-wavelength anomalous dispersion at 1.8 A resolution. It reveals a, homodimer, containing in each subunit a compact five-alpha-helix core that, is stabilized by four conserved disulfide bridges. rVI adopts a novel fold, that is likely conserved across the protein family, indicating a conserved, function. It shows no similarity to the Duffy-binding-like domains of, EBA-175 involved in erythrocyte binding, indicating a distinct role., Remarkably, rVI possesses structural features related to the KIX-binding, domain of the coactivator CREB-binding protein, supporting the binding and, trafficking roles that have been ascribed to it and providing a rational, basis for further experimental investigation of its function.
About this Structure
2RJI is a Single protein structure of sequence from Plasmodium falciparum with as ligand. Full crystallographic information is available from OCA.
Reference
Malarial EBA-175 region VI crystallographic structure reveals a KIX-like binding interface., Withers-Martinez C, Haire LF, Hackett F, Walker PA, Howell SA, Smerdon SJ, Dodson GG, Blackman MJ, J Mol Biol. 2008 Jan 18;375(3):773-81. Epub 2007 Nov 1. PMID:18036613
Page seeded by OCA on Wed Jan 23 11:03:59 2008
