2jbp
From Proteopedia
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PROTEIN KINASE MK2 IN COMPLEX WITH AN INHIBITOR (CRYSTAL FORM-2, CO-CRYSTALLIZATION)
Overview
The Ser/Thr protein kinase MAPKAP kinase 2 (MK2) plays a crucial role in, inflammation. We determined the structure of the kinase domain of MK2 in, complex with a low molecular mass inhibitor in two different crystal, forms, obtained from soaking and co-crystallization. To our knowledge, these are the first structures of MK2 showing the binding mode of an, inhibitor with high binding affinity (IC50 8.5 nM). The two crystal forms, revealed conformational flexibility in the binding site and extend the, experimental basis for rational drug design. Crystal form-1 contained one, MK2 molecule per asymmetric unit. Form-2 contained 12 molecules, which, arrange into two different types of MK2 trimers. One of them may serve as, a model for an intermediate state during substrate phosphorylation, as, each MK2 monomer places its activation segment into the substrate peptide, binding groove of the trimer neighbor.
About this Structure
2JBP is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Transferase, with EC number 2.7.11.1 Known structural/functional Sites: , , , , , , , , , and . Full crystallographic information is available from OCA.
Reference
Structural basis for a high affinity inhibitor bound to protein kinase MK2., Hillig RC, Eberspaecher U, Monteclaro F, Huber M, Nguyen D, Mengel A, Muller-Tiemann B, Egner U, J Mol Biol. 2007 Jun 8;369(3):735-45. Epub 2007 Mar 12. PMID:17449059
Page seeded by OCA on Wed Jan 23 11:12:51 2008
Categories: Homo sapiens | Single protein | Transferase | Eberspaecher, U. | Egner, U. | Hillig, R.C. | Huber, M. | Mengel, A. | Monteclaro, F. | Muller-Tiemann, B. | Nguyen, D. | P4O | Atp binding | Atp site | Co-crystallization | Kinase | Mapkap kinase 2 | Mk2 | Nucleotide-binding | Phosphorylation | Ser-thr kinase | Serine/threonine-protein kinase | Small molecule inhibitor
