3b5d
From Proteopedia
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EmrE multidrug transporter in complex with TPP, C2 crystal form
Overview
EmrE, a multidrug transporter from Escherichia coli, functions as a, homodimer of a small four-transmembrane protein. The membrane insertion, topology of the two monomers is controversial. Although the EmrE protein, was reported to have a unique orientation in the membrane, models based on, electron microscopy and now defunct x-ray structures, as well as recent, biochemical studies, posit an antiparallel dimer. We have now reanalyzed, our x-ray data on EmrE. The corrected structures in complex with a, transport substrate are highly similar to the electron microscopy, structure. The first three transmembrane helices from each monomer, surround the substrate binding chamber, whereas the fourth helices, participate only in dimer formation. Selenomethionine markers clearly, indicate an antiparallel orientation for the monomers, supporting a "dual, topology" model.
About this Structure
3B5D is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
X-ray structure of EmrE supports dual topology model., Chen YJ, Pornillos O, Lieu S, Ma C, Chen AP, Chang G, Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):18999-9004. Epub 2007 Nov, 16. PMID:18024586
Page seeded by OCA on Wed Jan 23 11:41:47 2008
