2d7f

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spinqualitylabelsall models 2d7f, resolution 2.31Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of A lectin from canavalia gladiata seeds complexed with alpha-methyl-mannoside and alpha-aminobutyric acid

Overview

BACKGROUND: Lectins are mainly described as simple carbohydrate-binding, proteins. Previous studies have tried to identify other binding sites, which possible recognize plant hormones, secondary metabolites, and, isolated amino acid residues. We report the crystal structure of a lectin, isolated from Canavalia gladiata seeds (CGL), describing a new binding, pocket, which may be related to pathogen resistance activity in ConA-like, lectins; a site where a non-protein amino-acid, alpha-aminobutyric acid, (Abu), is bound. RESULTS: The overall structure of native CGL and, complexed with alpha-methyl-mannoside and Abu have been refined at 2.3 A, and 2.31 A resolution, respectively. Analysis of the electron density maps, of the CGL structure shows clearly the presence of Abu, which was, confirmed by mass spectrometry. CONCLUSION: The presence of Abu in a plant, lectin structure strongly indicates the ability of lectins on carrying, secondary metabolites. Comparison of the amino acids composing the site, with other legume lectins revealed that this site is conserved, providing, an evidence of the biological relevance of this site. This new action of, lectins strengthens their role in defense mechanisms in plants.

About this Structure

2D7F is a Single protein structure of sequence from Canavalia gladiata with , , and as ligands. Full crystallographic information is available from OCA.

Reference

Structure of a lectin from Canavalia gladiata seeds: new structural insights for old molecules., Delatorre P, Rocha BA, Souza EP, Oliveira TM, Bezerra GA, Moreno FB, Freitas BT, Santi-Gadelha T, Sampaio AH, Azevedo WF Jr, Cavada BS, BMC Struct Biol. 2007 Aug 2;7:52. PMID:17683532

Page seeded by OCA on Wed Jan 23 11:56:09 2008