This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2jpp
From Proteopedia
|
Structural basis of RsmA/CsrA RNA recognition: Structure of RsmE bound to the Shine-Dalgarno sequence of hcnA mRNA
Overview
Proteins of the RsmA/CsrA family are global translational regulators in, many bacterial species. We have determined the solution structure of a, complex formed between the RsmE protein, a member of this family from, Pseudomonas fluorescens, and a target RNA encompassing the, ribosome-binding site of the hcnA gene. The RsmE homodimer with its two, RNA-binding sites makes optimal contact with an, 5'-(A)/(U)CANGGANG(U)/(A)-3' sequence in the mRNA. When tightly gripped by, RsmE, the ANGGAN core folds into a loop, favoring the formation of a, 3-base-pair stem by flanking nucleotides. We validated these findings by, in vivo and in vitro mutational analyses. The structure of the complex, explains well how, by sequestering the Shine-Dalgarno sequence, the, RsmA/CsrA proteins repress translation.
About this Structure
2JPP is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.
Reference
Molecular basis of messenger RNA recognition by the specific bacterial repressing clamp RsmA/CsrA., Schubert M, Lapouge K, Duss O, Oberstrass FC, Jelesarov I, Haas D, Allain FH, Nat Struct Mol Biol. 2007 Sep;14(9):807-13. Epub 2007 Aug 19. PMID:17704818
Page seeded by OCA on Wed Jan 23 13:31:57 2008
