DNA

Deoxyribonucleic acid or DNA is a molecule which is the carrier of genetic information in nearly all the living organisms. It contains the biological instructions for the development, survival and reproduction of organisms.

DNA is found in the nucleus of a cell where it is packaged into a compact form called a chromosome with the help of several proteins known as histones. It is also found in cell structures called mitochondria. However in case of prokaryotes DNA is not enclosed in a nucleus or a membrane but is present in the cytoplasm. The DNA in prokaryotes in generally circular and supercoiled without any histones. DNA stores genetic information as a sequence of nucleotides in special regions known as genes which are used to make proteins. The expression of genetic information into proteins is a two-stage process wherein the sequence of nucleotides in DNA is converted to a molecule called Ribonucleic acid or RNA by a process called transcription. RNA is used to make proteins by another process called translation. The human genome contains nearly 3 · 10⁹ bases with around 20,000 genes on 23 chromosomes. ^[1]

DNA was first discovered by the German biochemist Frederich Miescher in the year 1869.^[2] Based on the works of Erwin Chargaff, James Watson, Francis Crick, Maurice Wilkins and Rosalind Franklin, the structure of DNA was discovered in the year 1953. The structure of DNA is a : two complementary strands of polynucleotides that run in opposite directions and are held together by hydrogen bonds between them. This structure helps the DNA replicate itself during cell division and also for a single strand to serve as template during transcription. ^[1]

Features of a DNA Molecule

Double Helix

consists of two polynucleotide chains, . The in DNA is composed of of a which is a beta-D-2'- deoxyribose and a purine or a pyrimidine . The four types of bases are the two double ringed purine base and and the two single pyrimidine bases and .Each nucleotide in a DNA chain is linked to another via . There are four nucleotides in DNA. The sugar-phosphate backbone of the DNA is very regular owing to the phosphodiester linkage whereas the ordering of bases is highly irregular.^[3]

A C G T

Purines Pyrimidines

Complementary Bases

The two chains in a DNA are joined by hydrogen bonds between specific bases. Adenine forms base pairs with thymine and guanine with cytosine. This specific base pairing between and is known as the Watson-Crick base pairing. The specificity of hydrogen bonding between bases leads to complementarity in the sequence of nucleotides in the two chains. Thus in a strand of DNA the content of adenine is equal to that of thymine and the guanine content is equal to the cytosine content. In general DNA with higher GC content is more stable than the one with higher AT content owing to the stabilization due to base stacking interactions.

DNA denaturation and renaturation

A DNA double strand can be separated into two single strands by breaking the hydrogen bonds between them. This is known as DNA denaturation. Thermal energy provided by heating can be used to melt or denature DNA. Molecules with rich GC content are more stable and thus denature at higher temperatures compared to the ones with higher AT content. The melting temperature is defined as the temperature at which half the DNA strands are in double helical state and half are in random coil state.^[4] The denatured DNA single strands have an ability to renature and form double stranded DNA again.

Grooves

In a the between C_1'-N₁ branch off from one side of the base pair and do not lie opposite to each other. This results in unequally spaced sugar-phosphate backbones and gives rise to two grooves: the and the of different width and depth. The minor groove is at the O₂ side of base pair and the major groove is on the opposite side.The floor of major groove is filled with nitrogen and oxygen atoms that project inward whereas in the minor groove they project outward. The larger size of major groove allows for the binding of DNA specific proteins.^[5][3]

Tautomeric forms of bases

The hydrogen atoms on the bases move from nitrogen or oxygen atom on ring to another through shifts known as tautomeric shifts. However the hydrogens have preferred atomic locations. Based on the movement of hydrogen atoms the nitrogen atoms are in amino or imino configuration and the oxygen atoms are either in keto or enol forms. There is a preference for the amino and keto forms respectively which is very crucial for the biological functioning of DNA as it leads to the specificity in base pairing and thus complementarity of the chains.^[3]