2op9

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spinqualitylabelsall models 2op9, resolution 1.800Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Substrate Specificity Profiling and Identification of a New Class of Inhibitor for the Major Protease of the SARS Coronavirus

Overview

Severe acute respiratory syndrome (SARS) is an emerging infectious disease, associated with a high rate of mortality. The SARS-associated coronavirus, (SARS-CoV) has been identified as the etiological agent of the disease., Although public health procedures have been effective in combating the, spread of SARS, concern remains about the possibility of a recurrence., Various approaches are being pursued for the development of efficacious, therapeutics. One promising approach is to develop small molecule, inhibitors of the essential major polyprotein processing protease 3Clpro., Here we report a complete description of the tetrapeptide substrate, specificity of 3Clpro using fully degenerate peptide libraries consisting, of all 160 000 possible naturally occurring tetrapeptides. The substrate, specificity data show the expected P1-Gln P2-Leu specificity and elucidate, a novel preference for P1-His containing substrates equal to the expected, preference for P1-Gln. These data were then used to develop optimal, substrates for a high-throughput screen of a 2000 compound small-molecule, inhibitor library consisting of known cysteine protease inhibitor, scaffolds. We also report the 1.8 A X-ray crystal structure of 3Clpro, bound to an irreversible inhibitor. This inhibitor, an, alpha,beta-epoxyketone, inhibits 3Clpro with a k3/Ki of 0.002 muM-1 s-1 in, a mode consistent with the substrate specificity data. Finally, we report, the successful rational improvement of this scaffold with second, generation inhibitors. These data provide the foundation for a rational, small-molecule inhibitor design effort based upon the inhibitor scaffold, identified, the crystal structure of the complex, and a more complete, understanding of P1-P4 substrate specificity.

About this Structure

2OP9 is a Single protein structure of sequence from Sars coronavirus with as ligand. Full crystallographic information is available from OCA.

Reference

Substrate Specificity Profiling and Identification of a New Class of Inhibitor for the Major Protease of the SARS Coronavirus(,)., Goetz DH, Choe Y, Hansell E, Chen YT, McDowell M, Jonsson CB, Roush WR, McKerrow J, Craik CS, Biochemistry. 2007 Jul 3;. PMID:17605471

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