2o4u
From Proteopedia
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Crystal structure of Mammalian Dimeric Dihydrodiol Dehydrogenase
Overview
Dimeric dihydrodiol dehydrogenase (DD) catalyses the nicotinamide adenine, dinucleotide phosphate (NADP(+))-dependent oxidation of trans-dihydrodiols, of aromatic hydrocarbons to their corresponding catechols. This is the, first report of the crystal structure of the dimeric enzyme determined at, 2.0 A resolution. The tertiary structure is formed by a classical, dinucleotide binding fold comprising of two betaalphabetaalphabeta motifs, at the N-terminus and an eight-stranded, predominantly antiparallel, beta-sheet at the C-terminus. The active-site of DD, occupied either by a, glycerol molecule or the inhibitor 4-hydroxyacetophenone, is located in, the C-terminal domain of the protein and maintained by a number of, residues including Lys97, Trp125, Phe154, Leu158, Val161, Asp176, Leu177, Tyr180, Trp254, Phe279, and Asp280. The dimer interface is stabilized by a, large number of intermolecular contacts mediated by the beta-sheet of each, monomer, which includes an intricate hydrogen bonding network maintained, in principal by Arg148 and Arg202. Site-directed mutagenesis has, demonstrated that the intact dimer is not essential for catalytic, activity. The similarity between the quaternary structures of mammalian DD, and glucose-fructose oxidoreductase isolated from the prokaryotic organism, Zymomonas mobilis suggests that both enzymes are members of a unique, family of oligomeric proteins and may share a common ancestral gene., Proteins 2007. (c) 2007 Wiley-Liss, Inc.
About this Structure
2O4U is a Single protein structure of sequence from Macaca fascicularis with , and as ligands. Active as Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, with EC number 1.3.1.20 Full crystallographic information is available from OCA.
Reference
Structures of dimeric dihydrodiol dehydrogenase apoenzyme and inhibitor complex: Probing the subunit interface with site-directed mutagenesis., Carbone V, Endo S, Sumii R, Chung RP, Matsunaga T, Hara A, El-Kabbani O, Proteins. 2007 Jul 24;. PMID:17654552
Page seeded by OCA on Wed Jan 23 14:14:26 2008
