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2hqr
From Proteopedia
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Structure of a Atypical Orphan Response Regulator Protein Revealed a New Phosphorylation-Independent Regulatory Mechanism
Overview
Two-component signal transduction systems, commonly found in prokaryotes, typically regulate cellular functions in response to environmental, conditions through a phosphorylation-dependent process. A new type of, response regulator, hp1043 (HP-RR) from Helicobacter pylori, has been, recently identified. HP-RR is essential for cell growth and does not, require the well known phosphorelay scheme. Unphosphorylated HP-RR binds, specifically to its own promoter (P(1043)) and autoregulates the promoter, of the tlpB gene (P(tlpB)). We have determined the structure of HP-RR by, NMR and x-ray crystallography, revealing a symmetrical dimer with two, functional domains. The molecular topology resembles that of the OmpR/PhoB, subfamily, however, the symmetrical dimer is stable even in the, unphosphorylated state. The dimer interface, formed by three secondary, structure elements (alpha4-beta5-alpha5), resembles that of the active, phosphorylated forms of ArcA and PhoB. Several conserved residues of the, HP-RR dimeric interface deviate from the OmpR/PhoB subfamily, although, there are similar salt bridges and hydrophobic patches within the, interface. Our findings reveal how a new type of response regulator, protein could function as a cell growth-associated regulator in the, absence of post-translational modification.
About this Structure
2HQR is a Single protein structure of sequence from Helicobacter pylori. Full crystallographic information is available from OCA.
Reference
Structure of an Atypical Orphan Response Regulator Protein Supports a New Phosphorylation-independent Regulatory Mechanism., Hong E, Lee HM, Ko H, Kim DU, Jeon BY, Jung J, Shin J, Lee SA, Kim Y, Jeon YH, Cheong C, Cho HS, Lee W, J Biol Chem. 2007 Jul 13;282(28):20667-75. Epub 2007 May 9. PMID:17491010
Page seeded by OCA on Wed Jan 23 14:22:47 2008
