1w3k
From Proteopedia
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ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHAERENS IN COMPLEX WITH CELLOBIO DERIVED-TETRAHYDROOXAZINE
Overview
The understanding of transition state mimicry in glycoside hydrolysis is, increasingly important both in the quest for novel specific therapeutic, agents and for the deduction of enzyme function and mechanism. To aid, comprehension, inhibitors can be characterized through kinetic, thermodynamic, and structural dissection to build an "inhibition profile.", Here we dissect the binding of a tetrahydrooxazine inhibitor and its, derivatives, which display Ki values around 500 nm. X-ray structures with, both a beta-glucosidase, at 2 A resolution, and an endoglucanase at atomic, (approximately 1 A) resolution reveal similar interactions between the, tetrahydrooxazine inhibitor and both enzymes. Kinetic analyses reveal the, pH dependence of kcat/Km and 1/Ki with both enzyme systems, and ... [(full description)]
About this Structure
1W3K is a [Single protein] structure of sequence from [Bacillus agaradhaerens] with BGC, SO4, OXZ and GOL as [ligands]. Active as [Cellulase], with EC number [3.2.1.4]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structural, thermodynamic, and kinetic analyses of tetrahydrooxazine-derived inhibitors bound to beta-glucosidases., Gloster TM, Macdonald JM, Tarling CA, Stick RV, Withers SG, Davies GJ, J Biol Chem. 2004 Nov 19;279(47):49236-42. Epub 2004 Sep 8. PMID:15356002
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