2z39
From Proteopedia
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Crystal structure of Brassica juncea chitinase catalytic module Glu234Ala mutant (Bjchi3-E234A)
Overview
Brassica juncea chitinase is an endo-acting, pathogenesis-related protein, that is classified into glycoside hydrolase family 19, with highest, homology (50-60%) in its catalytic domain to class I plant chitinases., Here we report X-ray structures of the chitinase catalytic domain from, wild-type (apo, as well as with chloride ions bound) and a Glu234Ala, mutant enzyme, solved by molecular replacement and refined at 1.53, 1.8, and 1.7 A resolution, respectively. Confirming our earlier mutagenesis, studies, the active-site residues are identified as Glu212 and Glu234., Glu212 is believed to be the catalytic acid in the reaction, whereas, Glu234 is thought to have a dual role, both activating a water molecule in, its attack on the anomeric carbon, and stabilizing the charged, intermediate. The molecules in the various structures differ significantly, in the conformation of a number of loops that border the active-site, cleft. The differences suggest an opening and closing of the enzyme during, the catalytic cycle. Chitin is expected to dock first near Glu212, which, will protonate it. Conformational changes then bring Glu234 closer, allowing it to assist in the following steps. These observations provide, important insights into catalysis in family 19 chitinases.
About this Structure
2Z39 is a Single protein structure of sequence from Brassica juncea with as ligand. Active as Chitinase, with EC number 3.2.1.14 Full crystallographic information is available from OCA.
Reference
Crystal structures of a family 19 chitinase from Brassica juncea show flexibility of binding cleft loops., Ubhayasekera W, Tang CM, Ho SW, Berglund G, Bergfors T, Chye ML, Mowbray SL, FEBS J. 2007 Jul;274(14):3695-703. Epub 2007 Jul 2. PMID:17608716
Page seeded by OCA on Wed Jan 23 14:23:49 2008
