2dtv

From Proteopedia

Crystal structure of LysN, alpha-aminoadipate aminotransferase, from Thermus thermophilus HB27

Overview

The extremely thermophilic bacterium Thermus thermophilus HB27 synthesizes, lysine through alpha-aminoadipate (AAA). In this study, a T. thermophilus, gene encoding the enzyme that catalyses transamination of AAA was cloned, as a mammalian kynurenine/AAA aminotransferase (Kat2) gene homologue. A T., thermophilus mutant with disruption of the Kat2 homologue required a, longer lag phase for growth and showed slower growth in minimal medium., Furthermore, addition of AAA or lysine shortened the lag phase and, improved the growth rate. The Kat2 homologue was therefore termed lysN., LysN recognizes not only 2-oxoadipate, an intermediate of lysine, biosynthesis, but also 2-oxoisocaproate, 2-oxoisovalerate and, 2-oxo-3-methylvalerate, intermediates of leucine, valine and isoleucine, biosyntheses, respectively, along with oxaloacetate, a compound in the TCA, cycle, as an amino acceptor. These results suggest multiple roles of LysN, in several cellular metabolic pathways including lysine and branched-chain, amino acid biosyntheses.

About this Structure

2DTV is a Single protein structure of sequence from Thermus thermophilus with and as ligands. Active as 2-aminoadipate transaminase, with EC number 2.6.1.39 Full crystallographic information is available from OCA.

Reference

alpha-Aminoadipate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus., Miyazaki T, Miyazaki J, Yamane H, Nishiyama M, Microbiology. 2004 Jul;150(Pt 7):2327-34. PMID:15256574

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