2z8g

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Template:STRUCTURE 2z8g

Aspergillus niger ATCC9642 isopullulanase complexed with isopanose

Template:ABSTRACT PUBMED 18155243

About this Structure

2z8g is a 2 chain structure with sequence from Aspergillus niger. Full crystallographic information is available from OCA.

Reference

  • Mizuno M, Koide A, Yamamura A, Akeboshi H, Yoshida H, Kamitori S, Sakano Y, Nishikawa A, Tonozuka T. Crystal structure of Aspergillus niger isopullulanase, a member of glycoside hydrolase family 49. J Mol Biol. 2008 Feb 8;376(1):210-20. Epub 2007 Dec 5. PMID:18155243 doi:10.1016/j.jmb.2007.11.098
  • Akeboshi H, Tonozuka T, Furukawa T, Ichikawa K, Aoki H, Shimonishi A, Nishikawa A, Sakano Y. Insights into the reaction mechanism of glycosyl hydrolase family 49. Site-directed mutagenesis and substrate preference of isopullulanase. Eur J Biochem. 2004 Nov;271(22):4420-7. PMID:15560783 doi:10.1111/j.1432-1033.2004.04378.x
  • Akeboshi H, Kashiwagi Y, Aoki H, Tonozuka T, Nishikawa A, Sakano Y. Construction of an efficient expression system for Aspergillus isopullulanase in Pichia pastoris, and a simple purification method. Biosci Biotechnol Biochem. 2003 May;67(5):1149-53. PMID:12834298

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