2eg8

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spinqualitylabelsall models 2eg8, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

The crystal structure of E. coli dihydroorotase complexed with 5-fluoroorotic acid

Overview

Dihydroorotase (DHOase) catalyzes the reversible cyclization of, N-carbamyl-l-aspartate (CA-asp) to l-dihydroorotate (DHO) in the de novo, biosynthesis of pyrimidine nucleotides. DHOase is a potential, anti-malarial drug target as malarial parasites can only synthesize, pyrimidines via the de novo pathway and do not possess a salvage pathway., Here we report the structures of Escherichia coli DHOase crystallized, without ligand (1.7 A resolution) and in the presence of the inhibitors, 2-oxo-1,2,3,6-tetrahydropyrimidine-4,6-dicarboxylate (HDDP; 2.0 A) and, 5-fluoroorotate (FOA, 2.2 A). These are the first crystal structures of, DHOase-inhibitor complexes, providing structural information on the mode, of inhibitor binding. HDDP possesses features of both the substrate and, product, and ligates the Zn atoms in the active site. In addition, HDDP, forms hydrogen bonds to the flexible loop (residues 105-115) stabilizing, the "loop-in" conformation of the flexible loop normally associated with, the presence of CA-asp in the active site. By contrast, FOA, a, product-like inhibitor, binds to the active site in a similar fashion to, DHO but does not ligate the Zn atoms directly nor stabilize the loop-in, conformation. These structures define the necessary features for the, future design of improved inhibitors of DHOase.

About this Structure

2EG8 is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as Dihydroorotase, with EC number 3.5.2.3 Full crystallographic information is available from OCA.

Reference

Structures of Ligand-free and Inhibitor Complexes of Dihydroorotase from Escherichia coli: Implications for Loop Movement in Inhibitor Design., Lee M, Chan CW, Graham SC, Christopherson RI, Guss JM, Maher MJ, J Mol Biol. 2007 Jul 27;370(5):812-25. Epub 2007 May 22. PMID:17550785

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