2pnn
From Proteopedia
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Crystal Structure of the Ankyrin Repeat Domain of Trpv1
Overview
TRPV1 plays a key role in nociception, as it is activated by heat, low pH, and ligands such as capsaicin, leading to a burning pain sensation. We, describe the structure of the cytosolic ankyrin repeat domain (ARD) of, TRPV1 and identify a multiligand-binding site important in regulating, channel sensitivity within the TRPV1-ARD. The structure reveals a binding, site that accommodates triphosphate nucleotides such as ATP, and, biochemical studies demonstrate that calmodulin binds the same site., Electrophysiology experiments show that either ATP or PIP(2) prevent, desensitization to repeated applications of capsaicin, i.e., tachyphylaxis, while calmodulin plays an opposing role and is necessary, for tachyphylaxis. Mutations in the TRPV1-ARD binding site eliminate, tachyphylaxis. We present a model for the calcium-dependent regulation of, TRPV1 via competitive interactions of ATP and calmodulin at the, TRPV1-ARD-binding site and discuss its relationship to the C-terminal, region previously implicated in interactions with PIP(2) and calmodulin.
About this Structure
2PNN is a Single protein structure of sequence from Rattus norvegicus with as ligand. Full crystallographic information is available from OCA.
Reference
The Ankyrin Repeats of TRPV1 Bind Multiple Ligands and Modulate Channel Sensitivity., Lishko PV, Procko E, Jin X, Phelps CB, Gaudet R, Neuron. 2007 Jun 21;54(6):905-18. PMID:17582331
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