2iy9
From Proteopedia
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CRYSTAL STRUCTURE OF THE A-SUBUNIT OF THE AB5 TOXIN FROM E. COLI
Overview
AB5 toxins are produced by pathogenic bacteria and consist of enzymatic A, subunits that corrupt essential eukaryotic cell functions, and pentameric, B subunits that mediate uptake into the target cell. AB5 toxins include, the Shiga, cholera and pertussis toxins and a recently discovered fourth, family, subtilase cytotoxin, which is produced by certain Shiga toxigenic, strains of Escherichia coli. Here we show that the extreme cytotoxicity of, this toxin for eukaryotic cells is due to a specific single-site cleavage, of the essential endoplasmic reticulum chaperone BiP/GRP78. The A subunit, is a subtilase-like serine protease; structural studies revealed an, unusually deep active-site cleft, which accounts for its exquisite, substrate specificity. A single amino-acid substitution in the BiP target, site prevented cleavage, and co-expression of this resistant protein, protected transfected cells against the toxin. BiP is a master regulator, of endoplasmic reticulum function, and its cleavage by subtilase cytotoxin, represents a previously unknown trigger for cell death.
About this Structure
2IY9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
AB5 subtilase cytotoxin inactivates the endoplasmic reticulum chaperone BiP., Paton AW, Beddoe T, Thorpe CM, Whisstock JC, Wilce MC, Rossjohn J, Talbot UM, Paton JC, Nature. 2006 Oct 5;443(7111):548-52. PMID:17024087
Page seeded by OCA on Wed Jan 23 14:43:01 2008
Categories: Escherichia coli | Single protein | Beddoe, T. | Paton, A.W. | Paton, J.C. | Rossjohn, J. | Talbot, U.M. | Thorpe, C.M. | Whisstock, J.C. | Wilce, M.C.J. | Coli | Plasmid | Shiga | Toxin
