Prp19
From Proteopedia
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| 3lrv, resolution 2.60Å () | |||||||
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| Ligands: | |||||||
| Gene: | PRP19, PSO4, YLL036C (Saccharomyces cerevisiae) | ||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Introduction
PRP19 (precusor RNA processing 19)is a splicing factor which is essential for proper pre-mRNA processing.[1]Its presence is required prior to the first splicing step, in which the 5' splice site is cleaved and a lariat intron/3' exon intermediate is formed. Through various isolation methods, over 40 PRP genes have been identified[2] - many of which have very similar homology and motifs such as Zinc fingers, RNA binding motifs, ATP dependant helicase and a motif for the beta subunit of G protein.[3]. However, PRP19 had no homological similarities to any known sequences. The function of PRP proteins can be broken down into three main categories,those involved in the first chemical step, such as PRP19, those involved in the accumulation of splicing intermediates, and those involved in the formation of the lariat-intron product. [3]
Role in Splicing
Studies have found that PRP19 associates with the spliceosome around the time that the U4 snRNP disassociates from the complex.
References
- ↑ Tsai WY, Chow YT, Chen HR, Huang KT, Hong RI, Jan SP, Kuo NY, Tsao TY, Chen CH, Cheng SC. Cef1p is a component of the Prp19p-associated complex and essential for pre-mRNA splicing. J Biol Chem. 1999 Apr 2;274(14):9455-62. PMID:10092627
- ↑ Tarn WY, Hsu CH, Huang KT, Chen HR, Kao HY, Lee KR, Cheng SC. Functional association of essential splicing factor(s) with PRP19 in a protein complex. EMBO J. 1994 May 15;13(10):2421-31. PMID:8194532
- ↑ 3.0 3.1 Cheng SC, Tarn WY, Tsao TY, Abelson J. PRP19: a novel spliceosomal component. Mol Cell Biol. 1993 Mar;13(3):1876-82. PMID:8441419
