1uow

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spinqualitylabelsall models 1uow, resolution 1.04Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CALCIUM BINDING DOMAIN C2B

Overview

Synaptotagmin I has two tandem Ca(2+)-binding C(2) domains, which are, essential for fast synchronous synaptic transmission in the central, nervous system. We have solved four crystal structures of the C(2)B, domain, one of them in the cation-free form at 1.50 A resolution, two in, the Ca(2+)-bound form at 1.04 A (two bound Ca(2+) ions) and 1.65 A (three, bound Ca(2+) ions) resolution and one in the Sr(2+)-bound form at 1.18 A, (one bound Sr(2+) ion) resolution. The side chains of four highly, conserved aspartic acids (D303, D309, D363, and D365) and two main chain, oxygens (M302:O and Y364:O), together with water molecules, are in direct, contact with two bound Ca(2+) ions (sites 1 and 2). At higher Ca(2+), concentrations, the side chain of N333 rotates and cooperates with D309 to, ... [(full description)]

About this Structure

1UOW is a [Single protein] structure of sequence from [Rattus norvegicus] with CA, ACT and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Crystallographic identification of Ca2+ and Sr2+ coordination sites in synaptotagmin I C2B domain., Cheng Y, Sequeira SM, Malinina L, Tereshko V, Sollner TH, Patel DJ, Protein Sci. 2004 Oct;13(10):2665-72. Epub 2004 Aug 31. PMID:15340165

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