2cxc

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spinqualitylabelsall models 2cxc, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of archaeal transcription termination factor NusA

Overview

The transcription factor NusA functions in transcriptional regulation, involving termination in bacteria. A NusA homolog consisting of only the, two KH domains is widely conserved in archaea, but its function remains, unknown. We have found that Aeropyrum pernix NusA strongly binds to a, certain CU-rich sequence near a termination signal. Our crystal structure, of A. pernix NusA revealed that its spatial arrangement is quite similar, to that of the KH domains of bacterial NusA. Thus, we consider archaeal, NusA to have retained some functions of bacterial NusA, including the, ssRNA-binding ability. Remarkable structural differences between archaeal, and bacterial NusA exist at the interface with RNAP, in connection with, the different NusA-binding sites around the termination signals., Transcriptional termination in archaea could differ from all of the known, bacterial and eukaryal mechanisms, in terms of the combination of a, bacterial factor and a eukaryal-type RNAP.

About this Structure

2CXC is a Single protein structure of sequence from Aeropyrum pernix. Full crystallographic information is available from OCA.

Reference

Crystal structure and RNA-binding analysis of the archaeal transcription factor NusA., Shibata R, Bessho Y, Shinkai A, Nishimoto M, Fusatomi E, Terada T, Shirouzu M, Yokoyama S, Biochem Biophys Res Commun. 2007 Mar 30;355(1):122-8. Epub 2007 Jan 31. PMID:17288993

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