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205d

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Revision as of 15:46, 29 January 2008 by OCA (Talk | contribs)
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205d, resolution 2.640Å

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STRUCTURE OF AN RNA DOUBLE HELIX INCLUDING URACIL-URACIL BASE PAIRS IN AN INTERNAL LOOP

Overview

The crystal structure of the RNA dodecamer 5'-GGACUUUGGUCC-3' has been, determined from X-ray diffraction data to 2.6 A resolution. This oligomer, forms an asymmetric double helix in the crystal. Four consecutive, non-Watson-Crick base-pairs are formed in the middle of the duplex, including the first intrahelical U-U (or T-T) pairs observed in an, oligonucleotide crystal structure. Two different conformations of U-U, pairs are observed in the context of the surrounding sequence. One of, these pairs is highly twisted, allowing a bound water to bridge across, strands in the major groove. The crystal packing illustrates a new form of, RNA helix-helix interaction.

About this Structure

205D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Structure of an RNA double helix including uracil-uracil base pairs in an internal loop., Baeyens KJ, De Bondt HL, Holbrook SR, Nat Struct Biol. 1995 Jan;2(1):56-62. PMID:7719854

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