Turns in Proteins

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PDB ID 1abb

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Turns are classified as a type of secondary structure, and therefore they have an ordered, repetitive structure. This article describes β-turns and γ-turns and defines and illustrates their ordered, repetitive structures.

Contents

Beta Turns

There are several different classes[1] of β-turns with all types containing four residues, but each class has a different range of psi and phi values for the second and third residues. Pro and Gly are commonly found in positions two and three, respectively, because their unique side chains permit a sharp bend in the peptide chain. A hydrogen bond formed between the backbond atoms of residues one and four (i + 3) is the major force maintaining the conformation of the bend in the chain, but in one class a Pro in the third position has the cis configuration which maintains the sharp bend without the aid of a hydrogen bond.

Seven β-turns are shown as blue traces in myohemerytherin in the scene to the right (). In two cases one β-turn follows another one so the blue traces are longer, and five of the turns contain hydrogen bonds shown in magenta. Turns shown in without the side chains so that the backbone atoms can be seen. One can now clearly see that the hydrogen bonds are positioned between the first and the fourth residues of the turn and involve backbone atoms.

The (marked with yellow star) does not have a hydrogen bond, but it does have Pro in a cis configuration. of turn 5-8 with cis configuration marked with two halos, the bonds of both carbon and nitrogen project to the same side of the peptide bond. Observe that with the other peptide bonds they project to opposite sides.

The colors the spheres for the helices but not for the turns.

Examples

The β-turns shown below were cut from either myohemerytherin (2mhr.pdb) or glycogen phosphorylase chain A (1abb.pdb). Compare the shapes of the turns and observe the differences in the phi and psi values of the second and third residues. Checking the synchronize box will permit you to rotate all the turns by rotating any one of the turns with the mouse. Each applet has a green link which opens a scene showing the Pro in the cis configuration or the hydrogen bond. Notice that with the presence of the cis Pro that the backbone atoms of the first and fourth residues are not in position to form a hydrogen bond. Other green links reveal Ramachandran plots which show the phi and psi positions of the second and third residues.

Class I Class II Class III Class IV B
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PDB ID 2mhr 5-8.pdb

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   Residue 2: φ = -66°, ψ = -19°
   Residue 3: φ = -91°, ψ = -1°
    present
   

   Residue 2: φ = -56°, ψ = +126°
   Residue 3: φ = +78°, ψ = +1°
    present
   

   Residue 2: φ = , ψ =
   Residue 3: φ = °, ψ = °
    present
   

   Residue 2: φ = -135°, ψ = +112°
   Residue 3: φ = -63°, ψ = +163°
    at position 3
   
   

PDB ID 2mhr 67-70

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PDB ID 2mhr 88-91

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   Residue 2: φ = -70°, ψ = -25°
   Residue 3: φ = -109°, ψ = +29°
    present
   

   Residue 2: φ = °, ψ = °
   Residue 3: φ = °, ψ = °
    present
   

   Residue 2: φ = -89°, ψ = +142°
   Residue 3: φ = -76°, ψ = +135°
    at position 3
   

Gamma Turns

γ-turns consist of three residues and contain a hydrogen bond between residues one and three. There are only two classes [2] of γ-turns. The γ-turns are not as common as the β-turns.

Notes and References

  1. Characteristics of β-turn classes
  2. Characteristics of γ-turn classes

External Links

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