2b2c
From Proteopedia
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Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase
Overview
The polyamine synthesis enzyme spermidine synthase (SPDS) has been cloned, from the model nematode Caenorhabditis elegans. Biochemical, characterisation of the recombinantly expressed protein revealed a high, degree of similarity to other eukaryotic SPDS with the exception of a low, affinity towards the substrate decarboxylated S-adenosylmethionine (Km =, 110 microM) and a less pronounced feedback inhibition by the second, reaction product 5'-methylthioadenosine (IC50 = 430 microM). The C., elegans protein that carries a nematode-specific insertion of 27 amino, acids close to its N-terminus was crystallized, leading to the first X-ray, structure of a dimeric eukaryotic SPDS.
About this Structure
2B2C is a Single protein structure of sequence from Caenorhabditis elegans. Active as Spermidine synthase, with EC number 2.5.1.16 Full crystallographic information is available from OCA.
Reference
Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase., Dufe VT, Luersen K, Eschbach ML, Haider N, Karlberg T, Walter RD, Al-Karadaghi S, FEBS Lett. 2005 Nov 7;579(27):6037-43. Epub 2005 Oct 5. PMID:16226262
Page seeded by OCA on Tue Jan 29 18:14:48 2008
