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2bdz
From Proteopedia
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Mexicain from Jacaratia mexicana
Overview
Mexicain is a 23.7 kDa papain-like cysteine protease from the tropical, plant Jacaratia mexicana. Extracted as a mix of proteases from the latex, of the fruit, mexicain is isolated after cation-exchange chromatography as, the most abundant product. The purified product inhibited with E-64 was, crystallized by sitting-drop vapour diffusion in the presence of, ethanolamine. Cryoprotected crystals diffracted X-rays from a home source, to 1.98 A and belong to the monoclinic space group P2(1), with unit-cell, parameters a = 57.36, b = 90.45, c = 80.39 A, beta = 92.64 degrees . The, asymmetric unit contains four molecules of mexicain, with a corresponding, crystal volume per protein weight (V(M)) of 2.24 A(3) Da(-1) and a solvent, content of 45% by volume. A molecular-replacement model has been, determined and refinement is in progress.
About this Structure
2BDZ is a Single protein structure of sequence from Jacaratia mexicana with as ligand. Full crystallographic information is available from OCA.
Reference
Purification, crystallization and preliminary X-ray analysis of mexicain., Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:15502326
Page seeded by OCA on Tue Jan 29 18:22:29 2008
