Replication Termination Protein
From Proteopedia
Replication Termination Proteins
Rtp and Tus These polar fork bocking sites have been found in yeast, pea, frog and human genomes.
RTP
RTP is a DNA binding protein from Bacillus Subtilis that uses a helix-loop-helix binding motif. In solution it shows a symmetric structure typical of the winged helix loop helix family, with an unstructured end, first alpha helix , unstructured loop that is equivalent to the first beta sheet , helix loop helix structure (-), 2 beta sheets with a connecting loop that makes up the 'wing' structure and an additional long alpha helix involved in dimerisation .
Assymmetry of RTP in DNA-binding
: Contacts upstream with rtp dimer bound to A-site
: Contacts with phosphate backbone of downstream DNA
Residues binding in both RTP monomers: Gln15, Arg16, Leu35, Asn53, His54, Thr55, Tyr58, Arg59, His62, gln72 (RED) Residues binding in only wing down position: Lys14, Tyr33, Lys77, Gln83, Val86 (BLUE) Residues binding in only wing up position: Thr9, Lys36, Glu56 (GREEN)
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Michal Harel, Alexander Berchansky, Craig Mooney, Joel L. Sussman
