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2czt

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Revision as of 16:51, 29 January 2008 by OCA (Talk | contribs)
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Image:2czt.gif

2czt, resolution 2.00Å

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lipocalin-type prostaglandin D synthase

Overview

Lipocalin-type prostaglandin D synthase is the key enzyme for the, production of prostaglandin D(2), a potent endogenous somnogen, in the, brain. We cloned, produced, and crystallized the native enzyme and, selenomethionyl Cys(65)Ala mutants of the recombinant mouse protein by the, hanging drop vapor-diffusion method with both malonate and citrate as, precipitants. The native crystals obtained with malonate belong to, orthorhombic space group P2(1)2(1)2(1) with lattice constants a = 46.2, b, = 66.8, and c = 105.3 A. The selenomethionyl crystals obtained with, citrate belong to orthorhombic space group C222(1) with lattice constants, a = 45.5, b = 66.8, and c = 104.5 A. The native crystals diffracted beyond, 2.1 A resolution.

About this Structure

2CZT is a Single protein structure of sequence from Mus musculus. Active as Prostaglandin-D synthase, with EC number 5.3.99.2 Full crystallographic information is available from OCA.

Reference

Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme., Irikura D, Kumasaka T, Yamamoto M, Ago H, Miyano M, Kubata KB, Sakai H, Hayaishi O, Urade Y, J Biochem (Tokyo). 2003 Jan;133(1):29-32. PMID:12761195

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