Barnase
From Proteopedia
Barnase (BA, 'BActerial" "RiboNucleASE') is a bacterial ribonuclease synthesized and secreted by the bacterium Bacillus amyloliquefaciens. The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.
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3D Structures of Barnase
3kch, 2f4y – BA cross-linked
2f56 - BA cross-linked + urea
2f5w - BA cross-linked + thiourea
2f5m - BA cross-linked + Bromo-ethanol
1fw7, 1bnr – BA - NMR
2kf3 – BA (mutant) – NMR
2kf4, 1b2x, 1a2p, 1bni, 1bnj – BA
1yvs – BA domain swapped
1b20, 1b21, 1b2z, 1bne, 1bnf, 1bng, 1brh, 1bri, 1brj, 1brk, 1bns, 1brg, 1bsa, 1bsb, 1bsc, 1bsd, 1bse, 1ban, 1bao – BA (mutant)
2kf5, 2kf6, 1brn, 1rnb – BA + polynucleotide
BA + BST
3da7 - BA (mutant) + BST
2za4, 1brs, 1bgs – BA + BST
1x1u, 1x1w, 1x1x, 1x1y, 1b27, 1b2u – BA + BST (mutant)
1b3s, 1b2s - BA (mutant) + BST (mutant)
2c4b – BA/MCOEETI (mutant)
